novel rapid combined RP-HPLC stability method development and validation for antiviral hiv combinations lamivudine, tenofovir, doravirine in dosage form and its application to in vitro dissolution

In the analysis of pharmaceutical agents new sophisticated chromatographic methods have been utilized for quality control purpose. current scenario ample amount drugs and newer formulations are available intended in cure diseases. Diseases like HIV, AIDs , Hepatitis, other viral diseases requires their combinations. As a result this there is need analyse purposes. Here api-drugs Lamivudine LAM, tenofovir TEN, Doravirine DOR, has analysed by RP-HPLC method tablet dosage-forms. This developed analysis, these three combined forms rapid with very less analytes The concentration range linearity selected was 7.5 to 45 µg/ml LAM & Tenofovir where as DOR it 2.5 15 µg/ml. Wavelength estimation 269nm column used Acclaim 120 C-18 ( 250 mm x 4.6 mm, 5 µm id ).